Impossible Foods. has filed a patent for a method to purify protein from cells, aiming to minimize undesirable odors and flavors while increasing yield. The process involves lysing cells, clarifying the lysate, filtering, and pasteurizing the protein at a pH between 8.5 and 12.0. GlobalData’s report on Impossible Foods gives a 360-degree view of the company including its patenting strategy. Buy the report here.
According to GlobalData’s company profile on Impossible Foods, Edible protein hydrolysis was a key innovation area identified from patents. Impossible Foods's grant share as of January 2024 was 45%. Grant share is based on the ratio of number of grants to total number of patents.
Method for purifying protein with minimized odors and flavors
The patent application (Publication Number: US20240016182A1) describes a method for purifying protein from a plurality of cells. The method involves lysing the cells, clarifying the lysate, filtering it to obtain a protein composition, and optionally pasteurizing it, all at a pH between about 8.5 and 12.0. The filtering step can include microfiltration, ultrafiltration, diafiltration, or a combination thereof. The clarified lysate can be obtained by centrifugation to less than about 20% dry solids. The protein composition produced should contain at least about 35% of compounds larger than 5 kDa, with at least about 50% of the protein falling between 10 kDa and 200 kDa.
Another aspect of the patent application includes a method for purifying protein from cells by lysing them, filtering the lysate, and optionally pasteurizing the protein composition at a pH between about 8.5 and 12.0. The filtering step can involve ultrafiltration, diafiltration, or a combination thereof. The protein composition should have a buffering capacity of less than about 2.5 mmol NaOH per gram dry solids and should have low levels of H2S, with detectable amounts being controlled based on the presence or absence of L-cysteine. The patent also covers a protein composition produced by the described methods, with specific criteria for protein size distribution and H2S levels under different conditions.
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